Western Blot:Article Title: Pharmacologic inhibition of BMI1 exerts antitumor effects against MYCN-amplified neuroblastoma, with activation of the p53 pathway
Article Snippet: .. Antibodies used for Western blotting analysis Target protein Dilution Source Supplier Catalog number Predicted band size (kDa) BMI1 1:500 Mouse Santa Cruz sc- 390443 41 Caspase-3 1:1000 Rabbit CST 9662 35 Cleaved caspase-3 (Asp175) 1:1000 Rabbit CST 9664 17, 19 PARP, Cleaved PARP (Asp214) 1:1000 Rabbit CST 9542 116, 89 Actin 1:1000 Rabbit Sigma- Aldrich A5060 42 α-Tubulin 1:1000 Rabbit CST 2144 52 p53 1:1000 Rabbit CST 2527 53 p21 Waf1/Cip1 1:1000 Rabbit CST 2947 21 NOXA 1:1000 Rabbit CST 14766 10 BAX 1:1000 Rabbit CST 2772 20 BCL2 1:1000 Rabbit CST 4223 26 MCL1 1:1000 Rabbit CST 5453 40 Ubiquityl-Histone H2A (Lys119) 1:2000 Rabbit CST 8240 23 Phospho-histone H2A.X (Ser139) 1:1000 Rabbit CST 9718 15 Abbreviation: CST, Cell Signaling Technology Supplementary Table 4. .. Antibodies used for immunofluorescence analysis Target protein Dilution Source Supplier Catalog number Phospho-histone H2A.X (Ser139) 1:200 Rabbit CST 9718 53BP1 1:100 Rabbit CST 4937 Ki67 1:200 Mouse BD 550609 Abbreviations: CST, Cell Signaling Technology; BD, BD Biosciences Supplementary Table 5.
Article Title: Rapid antidepressant effect of single-bout exercise is mediated by adiponectin-induced APPL1 nucleus translocation in anterior cingulate cortex
Article Snippet: .. 127 128 Items Antibody Host Company Dilutio n Catalog # Western blotting - Primary antibody APPL1 Rabbit CST 1:1000 3858S GluR1 Rabbit CST 1:1000 13185S PSD95 Rabbit CST 1:1000 2507S Synapsin I Rabbit CST 1:1000 5297T Synaptophysin Rabbit CST 1:1000 5461S Histone 4 Mouse CST 1:1000 2935 Acetyl-Histone 4 (Lys12) Rabbit CST 1:1000 13944S Actin Rabbit CST 1:5000 4967S Tubulin Rabbit CST 1:5000 2144S GAPDH Rabbit CST 1:5000 5174 Western blotting - Second antibody Goat anti-rabbit IgG - CST 1:2000 7074 Goat anti-mouse IgG - CST 1:2000 7076 Immunofluorescence - Primary antibody GABA Rabbit Sigma 1:200 A2052 GAD67 Mouse Millipore 1: 200 MAB5406 CAMKII Rabbit Abcam 1: 200 ab5683 CAMKII Mouse Abcam 1: 200 ab171095 c-Fos Mouse Abcam 1:200 ab208942 c-Fos Rabbit Abcam 1: 200 ab214672 APPL1 Mouse Santa Cruz 1:300 sc-271909 AdipoR1 Rabbit Abcam 1:200 Ab70362 AdipoR1 Rabbit Bioss 1:200 Bs-0610R Goat anti-mouse, 488 - Abcam 1:200 ab150113 Goat anti-mouse, 568 - Abcam 1:200 ab175473 Immunofluorescence - Second antibody Goat anti-mouse, 568 - Thermofish er 1:200 A-11004 Goat anti-rabbit, 568 Thermofish er 1:200 A-11011 Goat anti-rabbit, 488 - Thermofish er 1:200 A-11008 129 130 131 Table S4. .. Virus information 132 133 Name Viral titers (vg/ml) Supplier and Catalog AAV2/9-CaMKIIa -GCaMP6s 2.7 ×1012 Brain VTA AAV2-Syn-DIO-hM3Dq-mCherry 2.7 ×1013 vg/ ml Addgene plasmid #44361 AAV2-Syn-DIO-hM4Di-mCherry 2.5 ×1013 vg/ml Addgene plasmid #44362 AAV2-hSyn-DIO-mCherry 1.5 × 1013 vg/ml Addgene plasmid #50459 AAV9-CMV-DIO-RFPmir30shRNA(AdipoR1) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV9-CMV-DIO-RFPmir30shRNA(AdipoR2) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV2- CaMKIIa -Cre 1.5 × 1013 vg/ml Addgene plasmid #105558 AAV9-CMV-DIO-RFPmir30shRNA(APPL1) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV9-CMV-DIO-RFPmir30shRNA (Control) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV9- CaMKII a -APPL1 1.0 × 1013 vg/ml WZ Biosciences Inc AAV9- CaMKII a -mCherry 1.0 × 1013 vg/ml WZ Biosciences Inc 134 135 136 Table S5.
Article Title: Biallelic BRCA2 variants induce premature ovarian insufficiency by impaired meiotic homologous recombination
Article Snippet: .. The antibodies used in this study 101 Target Dilution Host species Supplier Catalog number Mouse DDX4 1:600 (IF) Mouse Abcam, UK ab27591 Mouse cleaved PARP 1:200 (IF) Rabbit CST, USA 94885S Mouse SYCP3 1:500 (IF) Mouse Abcam, UK ab97672 Mouse SYCP1 1:500 (IF) Rabbit Abcam, UK ab15090 Mouse γH2AX 1:500 (IF) Mouse Millipore, USA 05-636 Mouse SYCP3 1:500 (IF) Rabbit Abcam, UK ab15093 Mouse RPA 1:200 (IF) Rabbit Abcam, UK ab76420 Mouse RAD51 1:100 (IF) Rabbit Thermofisher, USA PA5-27195 Mouse DMC1 1:100 (IF) Rabbit Proteintech, USA 13714-1-AP Mouse Ki67 1:2000 (IF) Rabbit Abcam, UK ab15580 Mouse CD45 1:200 (IF) Rabbit CST, USA #70257 Mouse PLZF 1:50 (IF) Mouse Santa Curz, USA sc-28319 Mouse 53BP1 1:800 (IF) Rabbit Novus biologicals, USA NB100-304 Mouse γH2AX 1:500 (IF) Mouse Santa Curz, USA sc-517348 Mouse RAD51 1:500 (IF) Rabbit Thermofisher, USA PA5-27195 Mouse STELLA 1 :40 (IF) Goat R&D systems, USA AF2566 Donkey anti-Rabbit Alexa Fluor Plus 488 1:800 (IF) Rabbit Thermofisher, USA A32790 Donkey anti-Mouse Alexa Fluor Plus 555 1:800 (IF) Mouse Thermofisher, USA A31570 Donkey anti-Goat Alexa Fluor Plus 488 1 :800 (IF) Goat Thermofisher, USA A32814 Mouse γH2AX (WB) 1:1000 (WB) Rabbit CST, USA 9718S Beta Actin (WB) 1:3000 (WB) Mouse Proteintech, USA 66009-1-Ig 102 S-13 Supplementary Table 4. ..
Immunofluorescence:Article Title: Rapid antidepressant effect of single-bout exercise is mediated by adiponectin-induced APPL1 nucleus translocation in anterior cingulate cortex
Article Snippet: .. 127 128 Items Antibody Host Company Dilutio n Catalog # Western blotting - Primary antibody APPL1 Rabbit CST 1:1000 3858S GluR1 Rabbit CST 1:1000 13185S PSD95 Rabbit CST 1:1000 2507S Synapsin I Rabbit CST 1:1000 5297T Synaptophysin Rabbit CST 1:1000 5461S Histone 4 Mouse CST 1:1000 2935 Acetyl-Histone 4 (Lys12) Rabbit CST 1:1000 13944S Actin Rabbit CST 1:5000 4967S Tubulin Rabbit CST 1:5000 2144S GAPDH Rabbit CST 1:5000 5174 Western blotting - Second antibody Goat anti-rabbit IgG - CST 1:2000 7074 Goat anti-mouse IgG - CST 1:2000 7076 Immunofluorescence - Primary antibody GABA Rabbit Sigma 1:200 A2052 GAD67 Mouse Millipore 1: 200 MAB5406 CAMKII Rabbit Abcam 1: 200 ab5683 CAMKII Mouse Abcam 1: 200 ab171095 c-Fos Mouse Abcam 1:200 ab208942 c-Fos Rabbit Abcam 1: 200 ab214672 APPL1 Mouse Santa Cruz 1:300 sc-271909 AdipoR1 Rabbit Abcam 1:200 Ab70362 AdipoR1 Rabbit Bioss 1:200 Bs-0610R Goat anti-mouse, 488 - Abcam 1:200 ab150113 Goat anti-mouse, 568 - Abcam 1:200 ab175473 Immunofluorescence - Second antibody Goat anti-mouse, 568 - Thermofish er 1:200 A-11004 Goat anti-rabbit, 568 Thermofish er 1:200 A-11011 Goat anti-rabbit, 488 - Thermofish er 1:200 A-11008 129 130 131 Table S4. .. Virus information 132 133 Name Viral titers (vg/ml) Supplier and Catalog AAV2/9-CaMKIIa -GCaMP6s 2.7 ×1012 Brain VTA AAV2-Syn-DIO-hM3Dq-mCherry 2.7 ×1013 vg/ ml Addgene plasmid #44361 AAV2-Syn-DIO-hM4Di-mCherry 2.5 ×1013 vg/ml Addgene plasmid #44362 AAV2-hSyn-DIO-mCherry 1.5 × 1013 vg/ml Addgene plasmid #50459 AAV9-CMV-DIO-RFPmir30shRNA(AdipoR1) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV9-CMV-DIO-RFPmir30shRNA(AdipoR2) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV2- CaMKIIa -Cre 1.5 × 1013 vg/ml Addgene plasmid #105558 AAV9-CMV-DIO-RFPmir30shRNA(APPL1) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV9-CMV-DIO-RFPmir30shRNA (Control) >1.0 × 1013 vg/ml WZ Biosciences Inc AAV9- CaMKII a -APPL1 1.0 × 1013 vg/ml WZ Biosciences Inc AAV9- CaMKII a -mCherry 1.0 × 1013 vg/ml WZ Biosciences Inc 134 135 136 Table S5.
Recombinase Polymerase Amplification:Article Title: Biallelic BRCA2 variants induce premature ovarian insufficiency by impaired meiotic homologous recombination
Article Snippet: .. The antibodies used in this study 101 Target Dilution Host species Supplier Catalog number Mouse DDX4 1:600 (IF) Mouse Abcam, UK ab27591 Mouse cleaved PARP 1:200 (IF) Rabbit CST, USA 94885S Mouse SYCP3 1:500 (IF) Mouse Abcam, UK ab97672 Mouse SYCP1 1:500 (IF) Rabbit Abcam, UK ab15090 Mouse γH2AX 1:500 (IF) Mouse Millipore, USA 05-636 Mouse SYCP3 1:500 (IF) Rabbit Abcam, UK ab15093 Mouse RPA 1:200 (IF) Rabbit Abcam, UK ab76420 Mouse RAD51 1:100 (IF) Rabbit Thermofisher, USA PA5-27195 Mouse DMC1 1:100 (IF) Rabbit Proteintech, USA 13714-1-AP Mouse Ki67 1:2000 (IF) Rabbit Abcam, UK ab15580 Mouse CD45 1:200 (IF) Rabbit CST, USA #70257 Mouse PLZF 1:50 (IF) Mouse Santa Curz, USA sc-28319 Mouse 53BP1 1:800 (IF) Rabbit Novus biologicals, USA NB100-304 Mouse γH2AX 1:500 (IF) Mouse Santa Curz, USA sc-517348 Mouse RAD51 1:500 (IF) Rabbit Thermofisher, USA PA5-27195 Mouse STELLA 1 :40 (IF) Goat R&D systems, USA AF2566 Donkey anti-Rabbit Alexa Fluor Plus 488 1:800 (IF) Rabbit Thermofisher, USA A32790 Donkey anti-Mouse Alexa Fluor Plus 555 1:800 (IF) Mouse Thermofisher, USA A31570 Donkey anti-Goat Alexa Fluor Plus 488 1 :800 (IF) Goat Thermofisher, USA A32814 Mouse γH2AX (WB) 1:1000 (WB) Rabbit CST, USA 9718S Beta Actin (WB) 1:3000 (WB) Mouse Proteintech, USA 66009-1-Ig 102 S-13 Supplementary Table 4. ..
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